Composite

Part:BBa_K3191101:Design

Designed by: Alexander Meyer   Group: iGEM19_UNebraska-Lincoln   (2019-10-16)


pBAD + AgrC + AgrA + GFP with Multiple Cloning Site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 569
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1848
    Illegal BsaI.rc site found at 1896
    Illegal BsaI.rc site found at 3159


Design Notes

Nucleotides are codon optimized for Escherichia coli K-12.

This part contains multiple restriction sites following AgrA for the insertion of SarA (BBa_K3191103) or other BioBricks.

Source

National Center for Biotechnology Information, U.S. National Library of Medicine (2019) Staphycoloccus aureus sarA gene, complete cds. NCBI [online] https://www.ncbi.nlm.nih.gov/nucleotide/U46541.1?report=genbank&log$=nuclalign&blast_rank=7&RID=FVNZXD37014 (Accessed June 10, 2019)

UniProt (2019) UniProtKB - O07911 (O07911_STAAU). UniProt [online] https://www.uniprot.org/uniprot/O07911 (Accessed June 12, 2019)

UniProt (2019) UniProtKB - P0A0I7 (AGRA_STAAU)). UniProt [online] https://www.uniprot.org/uniprot/P0A0I7 (Accessed June 12, 2019)

References

Srivastava, S. K., Rajasree, K., Fasim, A., Arakere, G., and Gopal, B. (2014) Influence of the AgrC-AgrA complex on the response time of Staphylococcus aureus quorum sensing. J. Bacteriol. 196(15), 2876-2888

Reyes, D., Andrey, D. O., Monod, A., Kelley, W. L., Zhang, G., and Cheung, A. L. (2011) Coordinated regulation by AgrA, SarA, and SarR to control agr expression in Staphylococcus aureus. J. Bacteriol. 193(21), 6020-6031